Antibody Pairs Screening Service

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Overview

SERVICE NO: TSS007

Paired antibodies are two different antibodies generated against the same target, but with different binding regions or epitopes. Differential epitope recognition allows for simultaneous binding of both antibodies to the same target, making Sandwich ELISAs possible. This assay is a powerful tool used to quantify target analyte from complex samples across a myriad of fields. One antibody acts to capture the target and the other acts to detect it. They are applied in sandwich ELISA, IP-WB, LF, TIA, and protein-protein interaction.

Selection of antibodies use the traditional methods based on the immunization of animal. Whether generating a monoclonal antibody pair, a polyclonal antibody pair, or a combination, BOT Bioscience's years of expertise in developing antibody pairs is at your disposal.

Service Details

Phase	Details	Timeline
Antigen generation	Gene synthesis and expression Peptide synthesis and coupling	2-4 weeks
Antibody generation	airing Formats: pAb/pAb; pAb/mAb; mAb/mAb Primary screening and sequencing Purification and labeling	8-12 weeks
Sandwich ELISA testing	Sandwich ELISA screen, all against all Final titration ELISA	4-6 weeks
Deliverables	Pre-immune serum Protein Antigen Antigen Affinity Purified Unlabeled Antibody Antigen Affinity Purified Biotinylated Antibody Hybridoma cell lines (mAb only) Validated sandwich ELISA repor	1 week