Baculovirus-Insect Expression Service
- Overview
- Service Details
- Service Highlights
- Related Services
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SERVICE NO: TSS041
Baculovirus - Insect Cell Expression System is an efficient eukaryotic protein expression platform. By combining insect cells with baculovirus vectors, this system can achieve high-level expression of complex eukaryotic proteins. This system can perform specific post-translational modifications on mammalian proteins, such as glycosylation, phosphorylation, and correct protein folding. It is particularly suitable for expressing structurally complex, functionally active membrane proteins, virus-like particles, and antibodies. One of the most notable features is its ability to accommodate large DNA molecules in the vector, enabling the production of high-molecular-weight proteins. It can also accommodate multiple gene insertions, allowing for the simultaneous expression of different proteins. Within this system, it has a strong post-translational modification (PTM) capability, including key processes such as glycosylation and acetylation. BOT Bioscience Biotechnology can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins.
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Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vectors Obtain the correct recombinant plasmid | 2 weeks |
Preparation of recombinant Bacmid and high titer virus | Isolate the recombinant bacmid DNA Obtain baculovirus Repeat the infection | 2 weeks |
Scale up expression and purification | Infect insect cells Purification of target protein | 1 week |
Quality Control | Testing of purity, concentration | 1 week |
Short lead time
Cost-effective, high expression level
Multiple expression systems
One-stop service: from gene to protein expression and purification
Large capacity: provides protein at milligram to gram levels
In addition, we also provide a variety of Related Services, including
E. Coli Protein Expression Service
Mammalian Transient Protein Expression Service
Yeast Expression Service
Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.
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