Baculovirus-Insect Expression Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS041

      Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. The baculovirus–insect cell expression system offers multifaceted Highlightss that set it apart from other expression systems. One of the most noteworthy Highlightss is its capacity to accommodate large DNA molecules within the vector, which enables the production of high-molecular-weight proteins. And it can house multiple gene insertions, allowing for the concurrent expression of different proteins. It has strong post-translational modification (PTM) capabilities within this system, encompassing essential processes such as glycosylation and acetylation. BOT Bioscience can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins.

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      Service Details

      Phase

      Procedure

      Timeline

      Plasmid construction

      Codon optimization; gene synthesis

      Ligation to expression vector

      Obtain the correct recombinant plasmi

      2 weeks

      Preparation of recombinant Bacmid and high titer virus

      Isolate recombinant bacmid DNA

      Obtain baculovirus

      Repeat the infection

      2 weeks

      Scale up expression and purification

      Infect insect cells

      Purification of target protein

      1 week

      Quality Control

      Testing of purity, concentration,

      1 week


      Service Highlights

      Short lead time

      Cost-effective, high expression level

      Multiple expression systems

      One-stop service: from gene to protein expression purification

      Large capacity: provides protein at milligram to gram levels

      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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