Baculovirus-Insect Expression Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS041

      Baculovirus - Insect Cell Expression System is an efficient eukaryotic protein expression platform. By combining insect cells with baculovirus vectors, this system can achieve high-level expression of complex eukaryotic proteins. This system can perform specific post-translational modifications on mammalian proteins, such as glycosylation, phosphorylation, and correct protein folding. It is particularly suitable for expressing structurally complex, functionally active membrane proteins, virus-like particles, and antibodies. One of the most notable features is its ability to accommodate large DNA molecules in the vector, enabling the production of high-molecular-weight proteins. It can also accommodate multiple gene insertions, allowing for the simultaneous expression of different proteins. Within this system, it has a strong post-translational modification (PTM) capability, including key processes such as glycosylation and acetylation. BOT Bioscience Biotechnology can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins.


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      Service Details

      Phase

      Procedure

      Timeline

      Plasmid construction

      Codon optimization; gene synthesis

      Ligation to expression vectors

      Obtain the correct recombinant plasmid

      2 weeks

      Preparation of recombinant Bacmid and high titer virus

      Isolate the recombinant bacmid DNA

      Obtain baculovirus

      Repeat the infection

      2 weeks

      Scale up expression and purification

      Infect insect cells

      Purification of target protein

      1 week

      Quality Control

      Testing of purity, concentration

      1 week


      Service Highlights

      Short lead time

      Cost-effective, high expression level

      Multiple expression systems

      One-stop service: from gene to protein expression anpurification

      Large capacity: provides protein at milligram to gram levels

      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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