Chicken Polyclonal Antibody Production Services

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS016

      Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs. Usually, rabbits, rats, goats, chickens, and guinea pigs are commonly used for the preparation of polyantibodies. Among them, egg yolk is a good source of highly specific antibodies against mammalian antigens because of the phylogenetic distance between birds and mammals. Compared to common mammalian lgG, chicken lgY antibody has a strong resistance to heat, acid, ionic and have certain enzyme resistance, it can be used as an ideal diagnostic reagent and a drug for prevention and treatment. 

      Abvigen using SPF level chicken, ensures the pure background and higher production of the antibody, by using latex Immuno amplification technology, acquire antigen with stronger immunogenicity, improve the immunization success possibility. Our comprehensive array of custom antibody services includes a customizable option for Chicken immunization, eggs collection, and titer analysis for antibody production, plus extension possibilities, and get chicken antibody with higher titer.

      1 Pre sales communication.jpg2 Receiving orders.jpg3 gene synthesis vector construction.jpg
      4 Protein expression  Peptide Synthesis.jpg鸡.jpg6 Antiserum testing.jpg
      7 antibody purification.jpg8 Antibody testing.jpg9 Antibody delivery.jpg


      Service Details

      Phase

      Details

      Timeline

      Antigen preparation

      Peptide design, synthesis, conjugation

      Protein expression & purification

      Other antigen

      2-4 weeks

      Immunization & ELISA titration

      Conventional or accelerated immunization protocol

      Immune2-5 chickens

      Collect eggs

      ELISA titration


      8-10 weeks

      Antibody Purification

      Protein A purification

      Protein G purification

      Protein A/G purification

      Affinity purification

      Antigen specific affinity purification

      1-2 weeks

      Validation

      ELISA: Guaranteed, >1:10,000

      Western Blot

      Optional: Application Validation Test, IHC, IF, FC

      1-2 weeks


      Service Highlights

      Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification.

      Flexible service portfolio: Different host species and purification method are available.

      Mature technology system - mature technology system and high experimental success rate.

      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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