E. Coli Protein Expression Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS043

      The eukaryotic expression system of Escherichia coli is one of the most widely used recombinant protein expression platforms in the field of biotechnology. This system uses Escherichia coli as the host, taking advantage of its clear genetic background, simple cultivation, and rapid reproduction. It can efficiently express exogenous genes. By optimizing the expression vector and induction conditions, a large amount of target protein can be obtained in a short period of time, and the expression level of some proteins can reach over 50% of the total protein of the bacteria. This system is particularly suitable for expressing proteins that do not require complex post-translational modifications and is the preferred solution for producing recombinant proteins, enzyme preparations, and vaccine antigens in both scientific research and industrial fields. Combined with the use of affinity tags, such as His-tag and GST, it can further simplify the subsequent purification process and significantly improve the efficiency of protein preparation.

      How to optimize soluble protein expression in E. coli is the focus and Highlights of our company. BOT-bioscience has extensive experience and is very professional in E. coli protein expression and purification. We can solve various problems in the process of protein expression to meet the needs of customers.


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      Service Details

      Phase

      Procedure

      Timeline

      Plasmid construction

      Codon optimization;  gene synthesis

      Ligation to expression vectors

      Obtain the correct recombinant plasmid

      2 weeks

      Transformation and strain screening

      Transform the recombinant plasmid to host cells

      Select single colony for small-scale induced expression

      Optimize the expression condition 

      1 week

      Target protein expression and purification

      Large-scale expression

      Protein purification

      1 week

      Inclusion body renaturation

      Refolding if the target protein is inclusion body

      1 week

      Quality Control

      Testing of purity, concentration, etc.  QC report is provided. 

      1 week


      Service Highlights

      Guaranteed yield: 1-3 mg purified protein.

      Guaranteed purity: >85% protein purity.

      Soluble protein: we prioritize proteins obtained from supernatant.

      Rich experience: many kinds of protein purification and protein experience

      High success rate: the success rate of expression is higher than 95%


      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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