E. Coli Protein Expression Service
- Overview
- Service Details
- Service Highlights
- Related Services
- Case Study
- How to order
SERVICE NO: TSS043
The eukaryotic expression system of Escherichia coli is one of the most widely used recombinant protein expression platforms in the field of biotechnology. This system uses Escherichia coli as the host, taking advantage of its clear genetic background, simple cultivation, and rapid reproduction. It can efficiently express exogenous genes. By optimizing the expression vector and induction conditions, a large amount of target protein can be obtained in a short period of time, and the expression level of some proteins can reach over 50% of the total protein of the bacteria. This system is particularly suitable for expressing proteins that do not require complex post-translational modifications and is the preferred solution for producing recombinant proteins, enzyme preparations, and vaccine antigens in both scientific research and industrial fields. Combined with the use of affinity tags, such as His-tag and GST, it can further simplify the subsequent purification process and significantly improve the efficiency of protein preparation.
How to optimize soluble protein expression in E. coli is the focus and Highlights of our company. BOT-bioscience has extensive experience and is very professional in E. coli protein expression and purification. We can solve various problems in the process of protein expression to meet the needs of customers.
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Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vectors Obtain the correct recombinant plasmid | 2 weeks |
Transformation and strain screening | Transform the recombinant plasmid to host cells Select single colony for small-scale induced expression Optimize the expression condition | 1 week |
Target protein expression and purification | Large-scale expression Protein purification | 1 week |
Inclusion body renaturation | Refolding if the target protein is inclusion body | 1 week |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Guaranteed yield: 1-3 mg purified protein.
Guaranteed purity: >85% protein purity.
Soluble protein: we prioritize proteins obtained from supernatant.
Rich experience: many kinds of protein purification and protein experience
High success rate: the success rate of expression is higher than 95%
In addition, we also provide a variety of Related Services, including
Baculovirus-Insect Expression Service
Mammalian Transient Protein Expression Service

Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.
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