ITRAQ/ TMT Labeling Quantitative Technique Service

SERVICE NO: TSS027

Mass spectrometry has proven to be a key enabling technology for the quantification of changes in the proteome, surpassing the more traditional gel-based methods. Different methodologies have been applied, each with individual benefits and drawbacks. Broadly speaking, two experimental strategies currently prevail: label-based and label-free approaches. Chemical labelling using isobaric reagents (predominantly isobaric tags for relative and absolute quantification (iTRAQ) and tandem mass tags (TMT)) is widely applied in relative quantification workflows. The key features of iTRAQ and TMT are their multiplexing capability (currently 4-plex and 8-plex for iTRAQ and 6-plex for TMT) and the simultaneous identification and relative quantification of peptides and proteins. Such features are attractive and contribute to their popularity.

Custom Package

BOT Bioscience iTRAQ/TMT technical services include protease digestion, peptide pricing, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis, etc.

Wide scope of application

High throughput

Reliable results

High sensitivity

Strong separation ability

High degree of automation

Service Process

After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results