Insect Cell Protein Co-Expression Service
- Overview
- Service Details
- Service Highlights
- Related Services
- Case Study
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Service Number: PSLSJ003
Multiprotein complexes are crucial in cellular processes such as transcription and signal transduction. Their functions depend on their own properties and interactions with partner proteins, and they have significant value in many fields. Co-expression to reconstruct multiple proteins is a commonly used method. Insect cell protein co-expression technology relies on the baculovirus expression vector system (BEVS), which can simultaneously express multiple foreign genes in insect cells. BEVS has the ability to process eukaryotic proteins, and insect cells have high biological safety, making it widely used in the production of complex proteins. It mainly realizes multi-gene expression through two methods: co-infection and co-expression.
BOT Bioscience provides professional services, offering one-stop solutions from experimental design to protein identification, meeting diverse needs and facilitating scientific research.
Phase | Procedure | Timeline |
Co-Expression Strategy Design | 1. Single polycistronic baculovirus (co-expression) 2. Multiple monocistronic baculoviruses (co-infection) 3. Combination of the two strategies | 1 weeks |
Vector Construction and Virus Preparation | 1. Gene cloning 2. Recombinant virus generation 3. Virus amplification and titer determination | 1-2 weeks |
Insect Cell Culture and Protein Expression | 1. Select appropriate cell lines and culture under 2. Virus infection and expression 3. Protein harvest and preliminary purification | 1 weeks |
Protein Purification and Identification | 1. Lyse cells by ultrasonic treatment/lysozyme, purify through affinity chromatography 2. Verify products by SDS-PAGE and Western blot | 1 weeks |
Efficient Co-Expression
Precise Regulation
Post-Translational Modification
High Biological Safety
Flexible Customization
In addition, we also provide a variety of Related Services, including
E. Coli Protein Expression Service - BOT Bioscience
Yeast Expression Service - BOT Bioscience
Baculovirus-Insect Expression Service - BOT Bioscience
References
Kanai, Y., Athmaram, T. N., Stewart, M., & Roy, P. (2013). Multiple large foreign protein expression by a single recombinant baculovirus: a system for production of multivalent vaccines. Protein expression and purification, 91(1), 77-84.
O'Reilly, D. R., Miller, L. K., & Luckow, V. A. (1994). Baculovirus expression vectors: a laboratory manual. Oxford University Press.
Teng, C. Y., Chang, S. L., van Oers, M. M., & Wu, T. Y. (2013). Enhanced protein secretion from insect cells by co-expression of the chaperone calreticulin and translation initiation factor eIF4E. Molecular biotechnology, 54(1), 68-78.
McKenzie, E. A., & Abbott, W. M. (2018). Expression of recombinant proteins in insect and mammalian cells. Methods, 147, 40-49.
Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.
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