Mammalian Cell Protein Co-Expression Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      Service Number: PSLSJ004

      Mammalian cell multi-protein expression refers to a technology that simultaneously expresses two or more proteins in mammalian cells, aiming to meet the needs for structural analysis of multi-protein complexes, functional research, and recombinant protein production. 

       

      BOT Bioscience offers professional Mammalian Cell Protein Co-Expression Service, tailored to address the complexities of co-expressing multiple proteins and assembling functional multi-subunit complexes—challenges that are critical for advanced research and biopharmaceutical development.


      Service Details

      Phase

      Steps

      Time-consuming

      Expression Vector Construction

      1. Vector selection and modification

      2. Multi-gene insertion

      2 weeks

      Cell Transfection and Screening

      1. Host cell selection

      2. Cell transfection

      3. Stable cell line construction

      2 weeks

      High-Expression Cell Screening

      1. Fluorescent screening

      2. Monoclonal purification

      3. Expression verification

      2-3 weeks

      Large-Scale Culture and Protein Purification

      1. Selection and optimization of the culture system

      2. Protein purification and identification

      2-3 weeks


      Service Highlights

      Protein Function Integrity

      Multi-Protein Co-Expression

      Efficient Screening System

      Strong Scalability

      Suitability for Complex Protein Expression



       

      In addition, we also provide a variety of Related Services, including

      E. Coli Protein Expression Service - BOT Bioscience

      Yeast Expression Service - BOT Bioscience

      Baculovirus-Insect Expression Service - BOT Bioscience

       

      References

      Assur, Z., Hendrickson, W. A., & Mancia, F. (2012). Tools for coproducing multiple proteins in mammalian cells. Methods in molecular biology (Clifton, N.J.), 801, 173–187.

      Qin, C., Xiang, Y., Liu, J., Zhang, R., et al. (2023). Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system. Nat. Commun. 14, 1500.

      Wenjun Guo, Mengmeng Wang, Lei Chen. (2022). A co-expression vector for baculovirus-mediated protein expression in mammalian cells. Biochemical and Biophysical Research Communications, Volume 594, 69-73.


      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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