Mammalian Transient Protein Expression Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS040

      More complex protein structures usually require particular post-translational modifications and co-factors to support their elaborate folding and enzymatic activity. E.coli expression system are unable to perform these modifications and lack essential eukaryotic co-factors. There appears to be a progressive increase in the application of mammalian cells for proteins production because of its highest level of protein processing, which is important for complete biological activity. The Overview of large-scale transient transfection has enabled the use of mammalian cells more attractive in terms of speed and ease of use, particularly for cell surface and secreted glycoproteins. BOT Bioscience enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.

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      Service Details

      Phase

      Procedure

      Timeline

      Plasmid construction

      Codon optimization;  gene synthesis

      Ligation to expression vector

      Obtain the correct recombinant plasmid

      2 weeks

      Small scale expression

      Transient transfect HEK293, CHO and other cells

      Detect expression products

      Optimization of transfection conditions

      1-2 weeks

      Scale up expression and purification

      Scale up the culture cells and transfect

      Optimal purification method

      1-2 weeks

      Quality Control

      Testing of purity, concentration, etc.  QC report is provided. 

      1 week


      Service Highlights

      Rapid expression of functional protein or antibody.

      Customization: quantity, purity, endotoxin, and other services are optional.

      Modifications: isotope labeling, protein biotinylation, and other services.

      Expression scale-up.

      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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