Mouse Monoclonal Antibody Production Service

Overview
Service Details
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Overview

SERVICE NO: TSS046

A mouse monoclonal antibody is a type of antibody produced by a single clone of immune cells derived from mice. After immunization, antibody-secreting B cells are immortalized by fusion to myeloma cell lines, and the resultant hybridomas are screened using a client-directed customizable protocol to select for the desired antigen-specific antibody. Mouse immunization and spleen fusion to create hybridoma cell lines are two phases of an entire procedure for developing, producing, and purifying monoclonal antibodies.

Abvigen's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production.

We perform and report in-process ELISA testing and send samples to customers for evaluation, allowing customers to select which animals to use for fusion and which cell lines to subclone, expand, and deliver.

Service Details

Phase	Details	Timeline
Antigen preparation	Antigen designn Antigen synthesis and expression	Abvigen provided: 1-2 business days Peptide synthesis: 2-3 weeks Protein Expression: 2-4 weeks
Immunization and ELISA titration	Conventional or accelerated immunization protocol ELISA Titer testing	9-10 weeks
Fusion and screening	Fusion clone screening by indirect ELISA Addition screening assays available upon request	4-5 weeks
Subcloning before production	Subclone screening	6-7 weeks
Production and purification	Small-scale antibody purification Isotype identification Additional screening and characterization assays available upon request Antibody purification Antibody validation by indirect ELISA Additional validation, scale-up, and antibody engineering available upon request Low-endotoxin and serum-free culturing are available on request, as well as high-volume production	5-6 weeks
Deliverables	2-5 ELISA positive clones Purified antibodies, 0.1-0.5 mg/clone Remaining Antigens Final Project Report
Labeling	We offer a wide variety of Custom Antibody Labeling Services options	1-2 weeks