Nucleic Acid Labeling Service
- Overview
- Service Details
- Service Highlights
- Related Services
- Case Study
- How to order
SERVICE NO: TSS030
In order to capture a single DNA or RNA chain, determine the existence of transcripts of target genes or monitor gene expression, and observe their exact functions, nucleic acid labeling technology provides researchers with a powerful diagnostic tool.
Custom Package
BOT Bioscience launched the nucleic acid labeling technology service platform to covalently bind common probes labeled with enzymes, biotins and fluorescent chemical groups to DNA or RNA through chemical reaction steps. The combination of reagents and nucleic acid molecules will not change the structure of nucleic acids, nor will it affect downstream hybridization applications. The labeled nucleic acid molecules can be used in a variety of experiments according to the needs of researchers.
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Probe | Enzyme; TAMRA; Dabcyl |
Hapten | Biotin; Digoxin |
Fluorescein | FITC; Cy（Cy3，Cy5，Cy5.5，Cy7） |
Chemical dye | CAL Fluor、ROX |
Colloidal gold label | Co-Au |
Method | Reagent | Mark | Probe | Site | ||
DNA | Enzyme | 5’- | T4 PNK | γ-32PrATP | ssDNA | 5’-OH |
3’- | TdT | dNTP | ssDNA | 3’-OH | ||
Nick Translation | DNA-pol I | dNTP | DNA | Random | ||
Primer Extension | Kelnow | dNTP | DNA | Random | ||
PCR | Taq Polymerase | dNTP | DNA | Random | ||
Chemical | Conjugate | EDC | Amines/Carboxylates/Phosphates | DNA | 5’-OH | |
Photoreaction | Nonspecific cross linker | Nucleobase | DNA | Random | ||
RNA | Enzyme | Reverse Transcription | Reverse Transcriptase | dNTP | cDNA | Random |
5’- | T4 PNK | γ-32PrATP | RNA | 5’-OH | ||
3’- | T4 RNA Ligase | [5’-32P]pCp | RNA | 3’-OH | ||
Chemical | Oxidation | Periodate | Amine/Hydrazide | RNA | 3’-OH | |
Conjugate | EDC | Amines/Carboxylates/Phosphates | RNA | 5’-OH | ||
Photoreaction | Nonspecific cross linker | Nucleobase | RNA | Random | ||
Stable
Well Characterized
Quick turnaround
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.
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