Protein Antibody Sequencing Service

Antibody protein sequencing offers unique advantages over classical antibody sequencing from hybridoma cells:

It makes antibody sequencing possible without any cell line. While classical sequencing is applicable to hybridomas or phage display, antibody protein de novo sequencing only requires a few micrograms of antibody protein. Thus, even when the hybridoma is lost, recovering your antibody is not an issue anymore! The sequence can be recovered directly from your antibody and can further be produced by transient or stable expression.

It allows to characterize posttranslational modifications (and thus to verify antibody integrity and effectiveness). This can even not be seen classical DNA antibody sequencing.

The method is adaptable to high-throughput sequencing. Thus, this approach is perfectly suitable for the sequencing of hundreds of antibodies.

Antibody protein sequencing can be applied to all formats of antibodies without species restriction. We can sequence IgA, IgM, IgG, IgY, Fab, scFv from mice, rats, rabbits.

BOT Bioscience service consist of variable region, variable plus leader region, as well as full-length heavy- and light-chain antibody sequencing for all species, isotypes, and allotypes. Purified monoclonal antibodies in multivalent forms can be sequenced with 100% coverage of the desired regions as well as excellent accuracy. We provide antibody sequencing with 100% accuracy and satisfaction guarantee to meet our customers' needs.