Rabbit Polyclonal Antibody Production Service

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS052

      Polyclonal antibodies are heterogeneous and bind to several different antigen epitopes. Rabbits are the most commonly used laboratory animals for the production of polyclonal antibodies. The rabbit offers many advantages over other species, such as adequate body size, easy procedure for the collection of large blood samples, excellent responsiveness to a wide variety of antigens, presence of a single primary IgG isotype, and reavailability of secondary reagents. Polyclonal antibody production is essential in research activity and the schedules and methodologies used to immunize rabbits and produce polyclonal antibodies continue to vary depending on the immunogen, the adjuvant, and the end purpose.

      If you cannot find an antibody from our extensive antibody catalog to suit your needs, then custom antibody service team may help. BOT Bioscience custom antibody production service offers gene/peptide/protein production technologies to suit your needs at affordable prices. From antigen design through to custom antibody generation, our expert scientists and talented technical support team have got you covered all the way.

      1 Pre sales communication.jpg

      2 Receiving orders.jpg

      3 gene synthesis vector construction.jpg

      4 Protein expression  Peptide Synthesis.jpg

      5 Animal immunity.jpg

      6 Antiserum testing.jpg

      7 antibody purification.jpg

      8 Antibody testing.jpg

      9 Antibody delivery.jpg


      Service Details

      Phase

      Details

      Timeline

      Antigen preparation

      Protein, recombinant or purified

      Peptide

      Small molecules

      Nucleic acid

      1-2 weeks

      Animal Immunization

      Control serum collection

      Injection

      Serum collection

      ELISA titer detection

      4-6 weeks

      Antibody Purification

      Protein A purification

      Protein G purification

      Protein A/G purification

      Affinity purification

      IgG purification

      1-2 weeks

      Antibody Verification

      WB positive

      ELISA >1:10,000 or 1:100,000


      Antibody Conjugation

      Biotin

      Horseradish peroxidase (HRP)

      Alkaline peroxidase

      Fluorochrom

      1-2 weeks


      Service Highlights

      Custom rabbit antibodies—optional protocol immunizing rabbits.

      Rabbit titer guarantee—rabbit antiserum titer of 1:10,000 to 1:100,000 as measured with our ELISA assay

      Full-service integration—complete set of services for antigen preparation and antibody purification 

      ELISA diagnostic assay shows highly specific reactivity and very low background.


      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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