Single B Cell Antibody Production Service

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Overview

SERVICE NO: TSS019

Nowadays, antibody drugs are increasingly important for the pharmaceutical industry across the globe. In contrast to polyclonal antibodies, a monoclonal antibody is derived from a single B cell parent clone and will only recognize a single epitope per antigen. With the development of antibody preparation techniques, single B cell isolation offers a fast, high-throughput method to generate a cell line producing your antibody of interest.

B cells are isolated from an immunized animal, sorted into single wells, lysed, and their genomes sequenced. Genes encoding desired proteins are cloned into a vector and transiently transfected into mammalian cells. Single B cell antibody production has many advantages such as short turnaround time, low hands-on inputs, and simple operation process and few rounds of cell culture. BOT Bioscience offers a high-throughput,full integrated platform for accelerating antibody discovery. And the method allows detection of those very rare antibodies orpotential drugs with unique properties that are exceptionally hard to find.

Service Details

Phase	Details	Timeline
Antigen Preparation	Protein, recombinant or purified Peptide Small molecules Nucleic acid	1-2 weeks
Immunization	5-10 BALB/c mouse DNA, Protein, Cells 1-2 months, maintain repertoire diversity	4-6 weeks
Plasma B Cells Isolation	Isolate plasma B cells from Spleen and Bone Marrow	1-2 weeks
Single B Cell Screening	Up to 2E6 plasma B cells are screened. Protein binding Cell-based binding Sorting positive micro-droplets	4-5 weeks
Single Cell Sequencing	Cell sequencing High throughput expression	1-2 weeks
Validation	Evaluate product quality ELISA titer detection	1-2 weeks