Stable Cell Line Construction Service

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Overview

SERVICE NO: TSS034

Cell line construction is the process of creating a large population of identical cells that contain the gene that, on translation, encodes the biologics (recombinant proteins, antibodies, fusion proteins, vaccines, etc.). When large quantities of recombinant protein are required, the construction of stable expression lines is recommended for production. Stable cell lines can be used for long-term continuous growth without significant changes in the expression levels of the target gene. The construction of stable cell line refers to the entry of foreign genes into recipient cells and integration into the chromosomes, allowing the target protein to be stably expressed on the surface of cell membrane. Stable cell line has been used in functional studies of genes, protein production and drug screening. BOT Bioscience has been engaged in protein expression for many years and has extensive experience in developing stable cell lines, we use lentiviral vector for efficient gene delivery and to achieve high integration rate for several proteins, which can effectively enhance the production capacity. And we can provide customers with a one-stop service from gene sequence to stable cell line delivery.

Service Details

Phase	Procedure
Cell Test	Cell viability, cell density, and mycoplasma
Construction of Lentiviral Expression Plasmid	Codon optimization Gene synthesis Construction of lentiviral expression plasmid
Lentivirus Packaging	Co-transfer of expression plasmid and auxiliary plasmid into host cells
Cell Pool Screening and Verification	Positive cells screening Detect cell pool expression level
Single-Clone Screening and Verification	Single cell planking and verification
Quality Control	Concentration; Purity; Contamination test