WB/IHC Guaranteed Mouse Monoclonal Antibody Services

      • Overview
      • Service Details
      • Service Highlights
      • Related Services
      • Case Study
      • How to order
      Overview

      SERVICE NO: TSS012

      Normally, a good antigen is a large, complex molecule with a molecular weight greater than 10kDa and when injected to animals is able to induce high levels of specific antibody. However, peptides are small molecules, when emulsified in adjuvant are able to elicit poor immune response. In many cases, the prepared antibodies are difficult to meet the requirements of scientific research. 

      BOT Bioscience can offer custom antibody services, and our goal is to provide the best antibody products and services which can suit your research needs. To avoid reproducible antibody preparation, BOT Bioscience offers custom guaranteed antibodies of WB, IHC, etc. which are applied to customer’s study. BOT Bioscience provides a one-stop solution for worldwide customers' antibody needs. Our antibody production services include recombinant protein expression, peptide design and synthesis, polyclonal and monoclonal antibody production, affinity purification, identification and labeling service. Our packages provide you desired antibodies that will be successful in basic applications or guaranteed to work in your assay.

      1Pre sales communication.jpg

      2 Receiving orders.jpg

      3 gene synthesisvector construction.jpg

      4 Protein expression Peptide Synthesis.jpg

      5 Animal immunity.jpg

      6 Antiserum testing.jpg

      7 antibody purification.jpg

      8 Antibody validation.jpg

      9 Antibody delivery.jpg


      Service Details

      Phase

      Details

      Timeline

      Antigen

      Preparation

      Peptide design

      Peptide synthesis

      Peptide conjugation

      Protein expression

      1-2 weeks or

      2-4 weeks

      Immunization & ELISA titration

      Conventional or accelerated immunization protocol

      ELISA Titer testing

      8-10 weeks

      Screening & Subcloning

      Fusion clone screening by indirect ELISA


      Addition screening assays available upon request

      Subclone screening

      10-12 weeks

      Production & Purification

      Small-scale antibody purification

      Isotype identification

      Hybridoma cell culture

      Antibody production

      1-2 weeks

      Validation

      ELISA

      Dot Blot

      Western Blot

      IHC (Immunohistochemistry)

      1-2 weeks


      Service Highlights

      One-stop - Provide one-stop antibody service from gene to antigen to antibody.

      Application - Can screen antibodies according to your application needs.

      Large-scale - Meet the needs of diagnostic mass production.

      Strict quality control - Provides ELISA, WB, IHC, IF, IP, ChIP and other basic test.

      Application guaranteed antibody - Custom guaranteed antibodies of WB, IHC, etc.


      Related Services
      Case Study
      How to order

      Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.

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