Yeast Expression Service
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SERVICE NO: TSS042
The yeast expression system is an efficient eukaryotic protein expression platform, combining the simplicity of the prokaryotic system with the protein processing capabilities of higher eukaryotic organisms. Using hosts such as Saccharomyces cerevisiae, this system can achieve the correct folding, disulfide bond formation, and basic glycosylation modification of exogenous proteins, and is particularly suitable for the expression of eukaryotic proteins that require post-translational modifications. With a clear genetic background, rapid growth characteristics, and mature gene manipulation tools, the yeast system has irreplaceable advantages in vaccine production, industrial enzyme preparations, and the preparation of complex eukaryotic proteins, serving as an important bridge connecting prokaryotic expression and mammalian cell expression. The common yeast expression hosts used for recombinant protein expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. In Abvigen, various host cells and vectors are available for yeast expression.
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Transformation and strain screening | Linearization of recombinant plasmid Transform hosts by electroporation Verify successful transformants by PCR Obtain high copy | 1-2 weeks |
Small test | Small scale expression screening | 2 weeks |
Scale up expression and purification | Optimize expression conditions Scale up culture Protein purification | |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 weeks |
Proprietary Technologies: promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location.
Humanized antibody production in the yeast expression system.
Streamlined Services: from gene synthesis, to the protein expression, antibody production, et al.
In addition, we also provide a variety of Related Services, including
E. Coli Protein Expression Service
Mammalian Transient Protein Expression Service
Baculovirus-Insect Expression Service

Contact us or send an email at info@bot-bioscience.com for project quotations and more detailed information.
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