Custom service
Custom Peptide Synthesis
The peptide is often the smallest line item in a project and the one that decides whether it works.
For antibody production, peptide purity and conjugation chemistry matter more than length. A crude peptide carrying deletion sequences will raise antibodies against the wrong epitope, and a poorly controlled conjugation ratio will bury the epitope against the carrier.
Peptides are synthesised by Fmoc solid-phase chemistry, purified by preparative HPLC and verified by ESI-MS. For immunisation we recommend a terminal cysteine for oriented maleimide coupling, and we report the measured conjugation ratio rather than a nominal one.
What you receive
- Lyophilised peptide with HPLC trace and mass spectrum.
- Purity options: ≥75% (screening), ≥95% (immunisation), ≥98% (assay standard).
- Optional KLH / BSA / OVA conjugation with measured coupling ratio.
- Hydrophobicity and solubility assessment before scale-up.
How the work runs
Sequence check
Hydrophobicity, aggregation risk and difficult couplings are reviewed before the quote is confirmed.
Synthesis
Fmoc SPPS on Rink amide or Wang resin, with double coupling at sterically hindered positions.
Cleavage & purification
TFA cleavage, ether precipitation, preparative HPLC to the requested purity band.
Verification & conjugation
ESI-MS confirmation, then maleimide or EDC coupling to the carrier protein of choice.
Specifications & timeline
- Scale
- 5 mg, 10 mg, 25 mg, 50 mg to gram scale
- Length
- Up to 60 residues routine
- Purity
- ≥75% / ≥95% / ≥98% by HPLC
- Modifications
- N-acetyl, amide C-terminus, FITC, biotin, phosphorylated residues
- Turnaround
- 2–3 weeks for standard lengths
Reagents to pair with it
- Recombinant ProteinsGrowth factors, cytokines and antigens expressed in E. coli, yeast, insect and mammalian platforms — 10,000+ proteins in stock.
- Native ProteinsPurified from natural sources to keep post-translational modifications and true conformational epitopes intact.
- Monoclonal AntibodiesRecombinant and hybridoma monoclonal antibodies validated for WB, IHC, IF, ELISA and flow cytometry.
Next step
Tell us the target, we will tell you what is feasible
Send the sequence, assay format and sensitivity you need. You will get a technical assessment — recommended platform, timeline and the risks worth knowing before you commit budget.