Scanning Electron Microscope (SEM) Detection

SERVICE NO:

The scanning electron microscope (SEM), simply referred to as the scanning electron microscope, uses secondary electron signals for imaging for observation and analysis of the surface or fracture morphology of samples. A scanning electron microscope emits an electron beam through an electron gun. Under the action of an accelerating voltage, the electron detector captures the electron signals on the surface of the sample in the sample chamber to form an image. The scanning electron microscope has the characteristics of high magnification, high resolution, simple sample preparation and convenient instrument operation.

In recent years, with the popularization and application of scanning electron microscopy technology, the observation of the ultrafine morphology of macromolecular particles by scanning electron microscopy has become an indispensable auxiliary research method for the comprehensive analysis of the structural characteristics of macromolecules. It has been increasingly applied in the observation and study of the surface morphology of biological macromolecules such as polysaccharides. Scanning electron microscopes can obtain images with high resolution, making them three-dimensional and realistic. In addition, SEM can also characterize the structure of purified polysaccharides and visually track the separation and purification of structurally stable polysaccharides, providing inspiration for the identification of the diversity and specificity of three-dimensional shapes of different polysaccharides.

BOT Bioscience has many years of experience in SEM detection and uses high-resolution SEM to extensively observe the microstructure on the surface of natural polysaccharides. By fully utilizing the characteristics of scanning electron microscopy such as high resolution, high magnification and strong stereoscopic effect, electron microscopy scanning observations were conducted on the polysaccharides obtained under different purification treatment conditions to search for the differences in ultramorphology among polysaccharide particles with different purification degrees, clarify the microstructure characteristic rules of polysaccharide particles, with the expectation of providing basic data and references for the in-depth study of polysaccharide structure.

Detection Process

Sample preparation - Sample processing -SEM observation - Data analysis - Delivery

Sample Requirements

1. Purity: The sample should be free of contaminants (residues of grease and solvents).

2. Size: Compatible sample stage (usually ¡Ü1 cm2, thickness ¡Ü5 mm);

3. Drying: The sample must be completely dehydrated to prevent vacuum damage from affecting imaging.

4. Conductivity: Non-conductive samples need to be gold-plated/platinum/carbon films to avoid the charging effect.

5. Flatness: The surface is flat or the cross-section is clear. If necessary, use a fixative to maintain the shape of the sample.

6. Stability: It can withstand electron beam irradiation, avoid damage or deformation, and undergo chemical cross-linking and other stabilization treatments when necessary.

Price

300 rmb per sample

Service Content

Table 1 Test modes and selection basis of SEM

Table 2 Test methods for different magnetic sample morphologies by SEM

Service Advantage

Advanced equipment: Equipped with a high-resolution scanning electron microscope, providing nano-level high-resolution imaging to clearly display the microstructure of polysaccharides;

Professional sample preparation: Based on the characteristics of polysaccharides, we offer professional sample fixation, drying and coating treatments to ensure imaging quality.

Comprehensive analysis: Provide high-precision image analysis, detailed data reports and image interpretation, including parameters such as particle size and porosity, to help customers deeply understand the structure of polysaccharides;

Customized services: Provide personalized detection solutions based on customer needs, and support combined services of SEM and other analytical technologies (such as FTIR).

Application Field

Materials science: Analyzing the application of polysaccharides in composite materials, such as reinforcing agents and fillers

Agricultural science: Analyzing the application of polysaccharides in soil improvement, such as water-retaining agents and soil structure modifiers

Food science: Studying the microstructure of polysaccharides in food, such as gums and cellulose, to help optimize the texture and taste of food

Cosmetics: Detect the morphology and distribution of polysaccharides in cosmetics, evaluate their moisturizing and stabilizing performance, and optimize cosmetic formulas through microstructure analysis

Biotechnology: Studying the microstructure changes of polysaccharides in enzymatic hydrolysis reactions and optimizing reaction conditions. The generation and structural changes of polysaccharides during microbial fermentation

Medicine: Analyze the microstructure of polysaccharides as drug carriers, optimize drug release performance, and study the application of polysaccharides in biomaterials, such as wound dressings and tissue engineering scaffolds

Service Process

After both parties reach an agreement through communication, the experimental plan is determined, the service requirements are set - the contract is signed - the advance payment is made - the experiment begins - the results are delivered