Proteomics
Proteomics is the large-scale study of proteomes. A proteome is a set of proteins produced in an organism, system, or biological context. The proteome is not constant; it differs from cell to cell and changes over time. To some degree, the proteome reflects the underlying transcriptome. However, protein activity (often assessed by the reaction rate of the processes in which the protein is involved) is also modulated by many factors in addition to the expression level of the relevant gene. Proteomics services and techniques can be applied to the analysis of a single protein or used for the large-scale analysis of a specific proteome including protein abundances, protein modifications, protein-protein interactions, and protein-compound interactions. BOT Bioscience‘s proteomics services team offers sample extraction, protein fractionation, peptidefractionation, protein mass spectrometry, protein identification, and protein quantification.
N-terminal Sequencing Service-Edman Degradation
N-terminal Edman degradation was developed by Edman and used to determine the N-terminal amino acid sequence of proteins or peptides as a classic biochemical method. And it is still widely used in protein sequence analysis. In the N-terminal Edman degradation, amino acids are gradually removed from the N-terminal of proteins through chemical reactions, and removed amino acid is identified by chromatography individually. N-terminal sequences is highly specificity, most proteins can be identified by sequencing few residues. Typically, 20-30 amino acids can be accurately determined. BOT Bioscience has extensive experience in analyzing the sequence of peptide and proteins. We provide N-terminal Edman degradation sequencing service for purified recombinant proteins, vaccines, peptides, antibodies. Using advanced instruments and optimized experimental processes to ensure high sensitivity and accuracy sequencing results.
N-terminal Sequencing Service-MS
N-terminal sequencing is a pivotal analytical method employed to determine the amino acid sequence at the N-terminus of a protein. The N-terminus, characterized by the free amino group at the protein's outset, plays a crucial role in governing the protein's biological function, stability, and subcellular localization. By elucidating the N-terminal sequence, it offers critical insights into the protein's structural features, post-translational modifications, and functional properties. BOT Bioscience can provide accurate sequencing results. The higher the purity of the customer's sample, the better. The report we deliver includes experimental steps, massspectrum parameters, details of N-terminal and C-terminal sequences of proteins, mass spectrum images and original data.
ITRAQ/ TMT Labeling Quantitative Technique Service
TMT/iTRAQ Quantitative Proteomics is a technique for the precise identification and quantification of all proteins expressed within a cell, organism or complex hybrid system. ITRAQ can use 8 different isotope reagents to simultaneously label and compare 8 different protein samples. These reagents are composed of report group, balance group and amine-specific reactive group. TMT can use up to 10 isotope labels to label the amino groups of polypeptides. After LC-MS/MS analysis, the relative content of proteins in 10 different samples can be compared at the same time. BOT Bioscience iTRAQ/TMT technical services include protease digestion, peptide pricing, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis, etc.
Label-free detection (also label-free (LF) technology, label-free analysis or label-free sensing) uses optics-based biosensors to convert biological binding responses into signals without using a fluorescent or any other detection label. A major advantage of label-free technology is that it allows the ligand to retain its native conformation and biological activity. This provides more physiologically relevant insights into the underlying biology than using a synthetically modified ligand. BOT Bioscience Label Free Technology Service application is oriented to disease marker screening, drug action target research, special function protein screening, pathogenesis and regulation mechanism research, action mechanism research, and plant stress resistance research.
Peptide Segment Coverage Detection Service
Peptide analysis is a main method to identify protein drugs and preparations. Peptide mapping can be used to decompose proteins by protease or chemical substances. Proper analytical methods can be used to identify the integrity and accuracy of primary structure of proteins, qualitative and quantitative analysis of protein degradation products. Peptide mapping has been used in many recombinant drugs as an important part of the primary structure confirmation and quality control. By using the existing mass spectrometry technology platform, BOT Bioscience provides peptide spectrogram analysis based on LC-MS/MS, which can be used to confirm whether the recombinant protein is fully expressed and detect whether the recombinant protein is broken during expression.
Amino Acid Sequencing Analysis Service
Amino acid analysis is a fundamental technique in biochemical research and drug development, providing crucial insights into the composition, structure, and function of proteins and peptides. By accurately determining the amino acid sequence and content, this method plays a pivotal role in understanding protein function, identifying potential therapeutic targets, and ensuring the quality and purity of pharmaceutical products. It can be utilized to assess the amino acid makeup of proteins, peptides, and protein drug samples, determine protein levels posthydrolysis, or characterize proteins through peptide mass fingerprinting or in conjunction with MS/Ms sequencing. BOT Bioscience employs GC/MS and LC/MS platforms to offer comprehensive amino acid analysis services, enabling both quantitative and qualitative analysis of over 70 amino acids and their derivatives.
Antibody Pairs Screening Service
Antibody pairs are two different antibodies generated against the same target that with different binding regions or epitopes. Differential epitope recognition allows both antibodies to bind to the same target simultaneously, making Sandwich ELISAs possible. Antibody pair can detect and quantify interaction molecules, ensure localization of sub-cellular, reduce the risk of missing weak or transient interactions, improve detection specificity and sensitivity. BOT Bioscience has years of expertise in developing antibody pairs, whether creating a monoclonal antibody pair, a polyclonal antibody pair, or a combination, we can provide based on your specific requirements and help you find out the most suitable matched pair for ELISA assay development.
