Antibody Fragmentation Kits
BOT Bioscience offers Antibody Fragmentation Kits that overcome the common challenges of over?digestion, poor purity, and lengthy workflows associated with traditional fragmentation methods. By converting intact IgG into monovalent Fab or bivalent F(ab′)? fragments, these enzyme?immobilized, spin?column?based solutions eliminate Fc?mediated background, improve tissue penetration, and support structural studies. The kits deliver rapid digestion control, high yields, and enzyme?free, functionally active fragments for applications including IHC, flow cytometry, epitope mapping, and antibody drug development.
Antibody Fragmentation Kits
BOT Bioscience offers two input quantity formats to accommodate both small-scale screening and medium-scale production needs:
-Standard kits (BIOK2024/BIOK2023 series): 25–250 μg IgG per sample; 10 antibody samples per kit
-Plus kits (BIOK2024P/BIOK2023P series): 0.25–4 mg IgG per sample; 10 antibody samples per kit
Product Name | Cat No. | Unit | Size |
Fab Preparation Kit | 25-250 ug | 10 antibody samples | |
0.25-4 mg | 10 antibody samples | ||
F(ab′)2 Preparation Kit | 25-250 ug | 10 antibody samples | |
0.25-4 mg | 10 antibody samples | ||
Mouse IgG1 Fab and F(ab′)2 Preparation Kit | 25-250 ug | 10 antibody samples | |
0.25-4 mg | 10 antibody samples | ||
Mouse IgG1 Fab Preparation Kit | 25-250 ug | 10 antibody samples | |
0.25-4 mg | 10 antibody samples | ||
Mouse IgG1 F(ab′)2 Preparation Kit | 25-250 ug | 10 antibody samples | |
0.25-4 mg | 10 antibody samples |
Applications
Fab fragments | Biomedical research (immunofluorescence, co-IP), diagnostic assays (lateral flow, ELISA), functional assays (receptor-ligand studies), industrial quality control, structural biology & epitope mapping (co-crystallization, X-ray crystallography) |
F(ab′)? fragments | Low-background immunoassays (IHC, IF, flow cytometry), sandwich ELISA, neutralization assays, in vivo isotype controls |
Mouse IgG1?specific Fab / F(ab′)? | Antibody development, functional blocking, isotype?matched negative controls; protective in preclinical models (e.g., HSV-2 prevention); enhanced tissue penetration due to smaller size |
Advantages
Immobilized enzyme technology – Instant reaction stop via centrifugation; enzyme?free, functionally active fragments
Target-specific design – Works with human, rabbit, and general mouse IgG; mouse IgG1 kits overcome the resistant subclass
High fragment quality – Retains strong antigen-binding activity, high purity, and yield
Simplified workflow – Prepacked spin columns, ready-to-use PBS buffers, minimal hands-on time
Stable storage – 4–8?°C ensures long-term reagent stability
Cost-effective pricing & 24/7 technical support response
For bulk orders, customized kit configurations, or technical support, please contact us at info@bot-bioscience.com. We look forward to assisting you in selecting the optimal fragmentation solution for your research.
